| 质粒名称: | pCAG Cas9-2A-Citrine |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92358 |
| 存储实验室: | Tatjana Sauka-Spengler |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4944 bp |
| 完整质粒大小: | 9886 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 2A Citrine |
| 物种来源: | -- |
| 插入大小: | 4944 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG Cas9-2A-Citrine was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 92358 ; http://n2t.net/addgene:92358 ; RRID:Addgene_92358) |
| 参考文献部分: | Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. 10.1242/dev.160333 PubMed 29386245 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAG Cas9-2A-Citrine 9886 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine
reporter. For CRISPR mediated gene knockouts in chicken embryos..
ACCESSION .
VERSION .
KEYWORDS pCAG Cas9-2A-Citrine
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9886)
AUTHORS Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA,
Sauka-Spengler T
TITLE Genome and epigenome engineering CRISPR toolkit for probing in vivo
cis-regulatory interactions in the chicken embryo
JOURNAL BioRxiv
REFERENCE 2 (bases 1 to 9886)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9886
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 5..384
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1674
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1598..1620
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1682..1701
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 1789..1798
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1801..1821
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1846..5946
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5947..5994
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 6010..6063
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 6070..6786
/codon_start=1
/product="enhanced variant of YFP (Heikal et al., 2001)"
/label=Citrine
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6115..6136)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6376..6395)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6723..6744
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(6878..6897)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 6943..6998
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(6997..7016)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(7389..7405)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7389..7405)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7402..7424)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7413..7429
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7437..7467)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7482..7503
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7562..7757
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 7608..7743
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7670..7689
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 7763..7897
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7800..7819)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7854..7873
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7965..7982)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8136..8724)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8216..8235)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8895..9755)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9518..9537
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9756..9860)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgct cgagagttcg
1741 aagccttaag tacgcgtagg cgcgccttaa ttaacgttta aacgctaggc caccatggcc
1801 ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc
1861 atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
1921 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
1981 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
2041 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
2101 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
2161 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
2221 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
2281 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
2341 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
2401 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
2461 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
2521 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
2581 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
2641 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
2701 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
2761 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
2821 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
2881 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
2941 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
3001 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
3061 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
3121 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
3181 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
3241 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
3301 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
3361 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
3421 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
3481 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
3541 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
3601 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
3661 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
3721 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
3781 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
3841 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
3901 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
3961 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
4021 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
4081 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
4141 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
4201 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
4261 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
4321 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
4381 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
4441 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
4501 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
4561 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
4621 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
4681 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
4741 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
4801 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
4861 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
4921 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
4981 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
5041 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
5101 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
5161 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
5221 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
5281 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
5341 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
5401 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
5461 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
5521 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
5581 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
5641 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
5701 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
5761 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
5821 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
5881 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
5941 ggcgacaaaa ggccggcggc cacgaaaaag gccggccagg caaaaaagaa aaaggatatc
6001 ggatcaggag agggcagggg aagtcttcta acatgcgggg acgtggagga aaatcccggg
6061 cccagatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
6121 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
6181 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
6241 tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac
6301 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
6361 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
6421 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
6481 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
6541 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
6601 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
6661 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
6721 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
6781 tacaagtaag gccgcaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg
6841 gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt
6901 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
6961 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
7021 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
7081 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
7141 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
7201 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
7261 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
7321 gatccctcga cctgcaccgt cgaccagctg gtcgacggtg caccgtcgac cagcttggcg
7381 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
7441 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
7501 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
7561 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
7621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
7681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
7741 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
7801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
7861 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
7921 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
7981 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
8041 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
8101 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
8161 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
8221 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
8281 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
8341 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
8401 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
8461 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
8521 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
8581 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
8641 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
8701 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
8761 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
8821 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
8881 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
8941 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
9001 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
9061 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
9121 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
9181 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
9241 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
9301 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
9361 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
9421 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
9481 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
9541 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
9601 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
9661 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
9721 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
9781 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
9841 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctg
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pAAVS1-P-CAG-GFP
- 下一篇：pcU6_3 sgRNA
